Rapid isolation, characterization, and glycan analysis of Cup a 1, the major allergen of Arizona cypress (Cupressus arizonica) pollen.
Alisi C, Afferni C, Iacovacci P, Barletta B, Tinghino R, Butteroni C, Puggioni EM, Wilson IB, Federico R, Schininà ME, Ariano R, Di Felice G, Pini C.
Alisi C, et al.
Allergy. 2001 Oct;56(10):978-84. doi: 10.1034/j.1398-9995.2001.103125.x.
Allergy. 2001.
PMID: 11576077
BACKGROUND: A rapid method for the purification of the major 43-kDa allergen of Cupressus arizonica pollen, Cup a 1, was developed. METHODS: The salient feature was a wash of the pollen in acidic buffer, followed by an extraction of the proteins and their purification by c …
BACKGROUND: A rapid method for the purification of the major 43-kDa allergen of Cupressus arizonica pollen, Cup a 1, was developed. M …