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Comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp. ancestrale -a wild ancestor of cultivated buckwheat.
Logacheva MD, Samigullin TH, Dhingra A, Penin AA. Logacheva MD, et al. BMC Plant Biol. 2008 May 20;8:59. doi: 10.1186/1471-2229-8-59. BMC Plant Biol. 2008. PMID: 18492277 Free PMC article.
The subject of our study, Fagopyrum esculentum, is a member of the family Polygonaceae belonging to the order Caryophyllales. ...The sequence was rapidly determined using a previously described approach that utilized a PCR-based method and employed universal …
The subject of our study, Fagopyrum esculentum, is a member of the family Polygonaceae belonging to the order Caryophyllales. ...The …
Stable transformation of the cotton plastid genome and maternal inheritance of transgenes.
Kumar S, Dhingra A, Daniell H. Kumar S, et al. Plant Mol Biol. 2004 Sep;56(2):203-16. doi: 10.1007/s11103-004-2907-y. Plant Mol Biol. 2004. PMID: 15604738 Free PMC article.
We employed a 'Double Gene/Single Selection (DGSS)' plastid transformation vector that harbors two selectable marker genes (aph A-6 and npt II) to detoxify the same antibiotic by two enzymes, irrespective of the type of tissues or plastids; by combining this with an …
We employed a 'Double Gene/Single Selection (DGSS)' plastid transformation vector that harbors two selectable marker genes (aph A
ASAP: amplification, sequencing & annotation of plastomes.
Dhingra A, Folta KM. Dhingra A, et al. BMC Genomics. 2005 Dec 7;6:176. doi: 10.1186/1471-2164-6-176. BMC Genomics. 2005. PMID: 16336644 Free PMC article.
Traditionally, the first step in mining the valuable information within a chloroplast genome requires sequencing a chloroplast plasmid library or BAC clones. ...Herein we present a universal high-throughput, rapid PCR-based technique to amplify, sequence and …
Traditionally, the first step in mining the valuable information within a chloroplast genome requires sequencing a chloroplast …
Rapid and accurate pyrosequencing of angiosperm plastid genomes.
Moore MJ, Dhingra A, Soltis PS, Shaw R, Farmerie WG, Folta KM, Soltis DE. Moore MJ, et al. BMC Plant Biol. 2006 Aug 25;6:17. doi: 10.1186/1471-2229-6-17. BMC Plant Biol. 2006. PMID: 16934154 Free PMC article.
The past five years have witnessed a dramatic increase in the number of completely sequenced plastid genomes, fuelled largely by advances in conventional Sanger sequencing technology. Here we report a further significant reduction in time and cost for plastid genome …
The past five years have witnessed a dramatic increase in the number of completely sequenced plastid genomes, fuelled largely by adva …
Resolving an ancient, rapid radiation in Saxifragales.
Jian S, Soltis PS, Gitzendanner MA, Moore MJ, Li R, Hendry TA, Qiu YL, Dhingra A, Bell CD, Soltis DE. Jian S, et al. Syst Biol. 2008 Feb;57(1):38-57. doi: 10.1080/10635150801888871. Syst Biol. 2008. PMID: 18275001
In contrast, parsimony analyses of different partitions yielded conflicting topologies, particularly with respect to the placement of Paeoniaceae, a clade characterized by a long branch. ...Age estimates indicate that the early diversification of Saxifragales occurr …
In contrast, parsimony analyses of different partitions yielded conflicting topologies, particularly with respect to the placement of Paeoni …
Comparative genomics analysis in Prunoideae to identify biologically relevant polymorphisms.
Koepke T, Schaeffer S, Harper A, Dicenta F, Edwards M, Henry RJ, Møller BL, Meisel L, Oraguzie N, Silva H, Sánchez-Pérez R, Dhingra A. Koepke T, et al. Plant Biotechnol J. 2013 Sep;11(7):883-93. doi: 10.1111/pbi.12081. Epub 2013 Jun 13. Plant Biotechnol J. 2013. PMID: 23763653 Free PMC article.
Prunus is an economically important genus with a wide range of physiological and biological variability. Using the peach genome as a reference, sequencing reads from four almond accessions and one sweet cherry cultivar were used for comparative analysis of these thr …
Prunus is an economically important genus with a wide range of physiological and biological variability. Using the peach genome as …
Comparative ultrastructure of fruit plastids in three genetically diverse genotypes of apple (Malus × domestica Borkh.) during development.
Schaeffer SM, Christian R, Castro-Velasquez N, Hyden B, Lynch-Holm V, Dhingra A. Schaeffer SM, et al. Plant Cell Rep. 2017 Oct;36(10):1627-1640. doi: 10.1007/s00299-017-2179-z. Epub 2017 Jul 11. Plant Cell Rep. 2017. PMID: 28698906 Free PMC article.
Recently, in a comparative study of the predicted plastid-targeted proteomes across seven plant species, we reported that each plant species is predicted to harbor a unique set of plastid-targeted proteins. ...Plastids transitioned from a proplastid-like plas …
Recently, in a comparative study of the predicted plastid-targeted proteomes across seven plant species, we reported that each plant …
Chloroplast genetic engineering via organogenesis or somatic embryogenesis.
Dhingra A, Daniell H. Dhingra A, et al. Methods Mol Biol. 2006;323:245-62. doi: 10.1385/1-59745-003-0:245. Methods Mol Biol. 2006. PMID: 16739583
Chloroplast genetic engineering offers a number of unique advantages, including high-level transgene expression, multigene engineering in a single transformation event, transgene containment via maternal inheritance, lack of gene silencing, position and pleiotropic …
Chloroplast genetic engineering offers a number of unique advantages, including high-level transgene expression, multigene engineerin …
An Arabidopsis mutant with high cyclic electron flow around photosystem I (hcef) involving the NADPH dehydrogenase complex.
Livingston AK, Cruz JA, Kohzuma K, Dhingra A, Kramer DM. Livingston AK, et al. Plant Cell. 2010 Jan;22(1):221-33. doi: 10.1105/tpc.109.071084. Epub 2010 Jan 15. Plant Cell. 2010. PMID: 20081115 Free PMC article.
Crossing hcef1 with pgr5, which is deficient in the antimycin A-sensitive pathway for plastoquinone reduction, resulted in a double mutant that maintained the high CEF1 phenotype, implying that the PGR5-dependent pathway is not involved. By contrast, crossing hcef1 …
Crossing hcef1 with pgr5, which is deficient in the antimycin A-sensitive pathway for plastoquinone reduction, resulted in a d …
In vitro cultures and regeneration of Bienertia sinuspersici (Chenopodiaceae) under increasing concentrations of sodium chloride and carbon dioxide.
Rosnow J, Offermann S, Park J, Okita TW, Tarlyn N, Dhingra A, Edwards GE. Rosnow J, et al. Plant Cell Rep. 2011 Aug;30(8):1541-53. doi: 10.1007/s00299-011-1067-1. Epub 2011 Apr 8. Plant Cell Rep. 2011. PMID: 21476090
To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. ...CO(2) enrichment during growth of RNS during Stage 3 had a large effe …
To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia …
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