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Directed enzyme evolution.
Farinas ET, Bulter T, Arnold FH. Farinas ET, et al. Curr Opin Biotechnol. 2001 Dec;12(6):545-51. doi: 10.1016/s0958-1669(01)00261-0. Curr Opin Biotechnol. 2001. PMID: 11849936 Review.
Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group.
Shim da J, Nemeria NS, Balakrishnan A, Patel H, Song J, Wang J, Jordan F, Farinas ET. Shim da J, et al. Among authors: farinas et. Biochemistry. 2011 Sep 6;50(35):7705-9. doi: 10.1021/bi200936n. Epub 2011 Aug 10. Biochemistry. 2011. PMID: 21809826 Free PMC article.
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