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2003 1
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Page 1
Suitability of the hemi-zona assay for the evaluation of the effect of the length of the equilibration period before cryopreservation.
Gürler H, Podhajsky E, Özen D, Leiding C, Bollwein H, Meinecke-Tillmann S. Gürler H, et al. Among authors: bollwein h. Theriogenology. 2018 Jan 15;106:157-163. doi: 10.1016/j.theriogenology.2017.10.021. Epub 2017 Oct 14. Theriogenology. 2018. PMID: 29059603
Spermatological analyses (progressive motile sperm (PMS), plasma membrane- and acrosome-intact sperm (PMAI), sperm showing a high degree of DNA fragmentation (%DFI)) were performed after 0 and 3 h of incubation after thawing. After an equilibration time of 48 h and …
Spermatological analyses (progressive motile sperm (PMS), plasma membrane- and acrosome-intact sperm (PMAI), sperm showing a high degree of …
NMR spectroscopy of a single mammalian early stage embryo.
Sivelli G, Conley GM, Herrera C, Marable K, Rodriguez KJ, Bollwein H, Sudano MJ, Brugger J, Simpson AJ, Boero G, Grisi M. Sivelli G, et al. Among authors: bollwein h. J Magn Reson. 2022 Feb;335:107142. doi: 10.1016/j.jmr.2021.107142. Epub 2021 Dec 31. J Magn Reson. 2022. PMID: 34999310 Free article.
The embryos in this study are obtained through In Vitro Fertilization (IVF) techniques, transported cryopreserved to the NMR laboratory, and measured shortly after thawing. In less than 1 h these spherical samples of just 130-190 m produce distinct spectral peaks, largely …
The embryos in this study are obtained through In Vitro Fertilization (IVF) techniques, transported cryopreserved to the NMR laboratory, and …
Effects of intravenous infusion of E. coli lipopolysaccharide in early pregnant cows.
Herzog K, Debertolis L, Kastelic JP, Schmicke M, Ulbrich SE, Bollwein H. Herzog K, et al. Among authors: bollwein h. Reproduction. 2019 Jan;157(1):65-76. doi: 10.1530/REP-18-0174. Reproduction. 2019. PMID: 30390615 Free article.
In LPS-treated cows, luteal area decreased (from 4.1 to 3.1 cm2; P 0.05) within 6 h and until 48 h. Luteal blood flow decreased by 39% (P 0.05) within the first 6 h after LPS, but returned to pre-treatment values by 48 h. Plasma P4 decreased by 62% (P …
In LPS-treated cows, luteal area decreased (from 4.1 to 3.1 cm2; P 0.05) within 6 h and until 48 h. Luteal blood flow decrease …
The sperm chromatin structure assay does not detect alterations in sperm chromatin structure induced by hydrogen peroxide.
Bittner-Schwerda L, Malama E, Siuda M, van Loon B, Bollwein H. Bittner-Schwerda L, et al. Among authors: bollwein h. Anim Reprod Sci. 2022 Dec;247:107094. doi: 10.1016/j.anireprosci.2022.107094. Epub 2022 Oct 17. Anim Reprod Sci. 2022. PMID: 36288681
The objective of this study was to investigate the effects of hydrogen peroxide (H(2)O(2)) on the chromatin structure of sperm. For this purpose, 44 cryopreserved bovine ejaculates were analyzed immediately post-thaw (control sperm, CON S), after 1 h of post-thaw in …
The objective of this study was to investigate the effects of hydrogen peroxide (H(2)O(2)) on the chromatin structure of sperm. For t …
Seasonal changes of DNA fragmentation and quality of raw and cold-stored stallion spermatozoa.
Wach-Gygax L, Burger D, Malama E, Bollwein H, Fleisch A, Jeannerat E, Thomas S, Schuler G, Janett F. Wach-Gygax L, et al. Among authors: bollwein h. Theriogenology. 2017 Sep 1;99:98-104. doi: 10.1016/j.theriogenology.2017.05.025. Epub 2017 May 30. Theriogenology. 2017. PMID: 28708505 Free article.
In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca(2+) level were determined in cold-stored semen (24 h, 48 h). SCSA was performed to assess mean DFI, SD of DFI and % DFI in raw frozen-thawed as well …
In addition, the percentages of sperm with intact plasma membrane and acrosome (PMAI %) and with low intracellular Ca(2+) level were determi …
Liposomes for cryopreservation of bovine sperm.
Röpke T, Oldenhof H, Leiding C, Sieme H, Bollwein H, Wolkers WF. Röpke T, et al. Among authors: bollwein h. Theriogenology. 2011 Nov;76(8):1465-72. doi: 10.1016/j.theriogenology.2011.06.015. Epub 2011 Aug 6. Theriogenology. 2011. PMID: 21820724
Sperm was incubated in the presence of liposomes for either 6 or 24 h at 4 C prior to freezing. Postfreeze survival rates were determined based on the percentage of progressively motile cells as well as the percentage of acrosome- and plasma membrane-intact cells. ...
Sperm was incubated in the presence of liposomes for either 6 or 24 h at 4 C prior to freezing. Postfreeze survival rates were determ …
3D histopathology of human tumours by fast clearing and ultramicroscopy.
Sabdyusheva Litschauer I, Becker K, Saghafi S, Ballke S, Bollwein C, Foroughipour M, Gaugeler J, Foroughipour M, Schavelová V, László V, Döme B, Brostjan C, Weichert W, Dodt HU. Sabdyusheva Litschauer I, et al. Among authors: bollwein c. Sci Rep. 2020 Oct 19;10(1):17619. doi: 10.1038/s41598-020-71737-w. Sci Rep. 2020. PMID: 33077794 Free PMC article.
Importantly, the sequence a-c is fully reversible, allowing the morphological correlation of one and the same histological structures, once visualized with our novel technique and once visualized by standard H&E- and IHC-staining. After reverting the clearing procedure …
Importantly, the sequence a-c is fully reversible, allowing the morphological correlation of one and the same histological structures, once …
Identification of genes associated with susceptibility to persistent breeding-induced endometritis by RNA-sequencing of uterine cytobrush samples.
Elshalofy A, Wagener K, Weber K, Blanco M, Bauersachs S, Bollwein H. Elshalofy A, et al. Among authors: bollwein h. Reprod Biol. 2022 Jun;22(2):100577. doi: 10.1016/j.repbio.2021.100577. Epub 2021 Dec 6. Reprod Biol. 2022. PMID: 34883452
Susceptibility to PBIE was evaluated by the presence of 2 cm of intrauterine fluid 24 h after AI, besides the fertility was determined by a sonographic pregnancy diagnosis 2 weeks after ovulation. ...
Susceptibility to PBIE was evaluated by the presence of 2 cm of intrauterine fluid 24 h after AI, besides the fertility was determine …
Evaluation of endometrial echotexture and cervical cytology in cows during and after treatment of endometritis.
Küçükaslan I, Kaya D, Emre B, Bollwein H, Ozyurtlu N, Mülazımoğlu SB, Aslan S. Küçükaslan I, et al. Among authors: bollwein h. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2014;42(6):343-50. doi: 10.15653/TPG-140473. Epub 2014 Nov 18. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2014. PMID: 25405281

In contrast, CON values of group E2 were lower (p < 0.05) at 0 h compared to other timepoints of examination and lower than in group C. HOM values were lower (p < 0.05) in groups E1, E2 and E3 than in group C on d 5 and d 10. HOM values were higher at 1 h comp

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In contrast, CON values of group E2 were lower (p < 0.05) at 0 h compared to other timepoints of examination and lower than in gro

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46 results