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Development of flow cytometry based adherence assay for Neisseria gonorrhoeae using 5'-carboxyfluorosceinsuccidyl ester.
Thakur SD, Obradovic M, Dillon JR, Ng SH, Wilson HL. Thakur SD, et al. BMC Microbiol. 2019 Mar 25;19(1):67. doi: 10.1186/s12866-019-1438-2. BMC Microbiol. 2019. PMID: 30909866 Free PMC article.
A flow cytometry based method, using the cell-permeable fluorescent dye 5'-carboxyfluoroscein succidyl ester (CFSE), was developed to dramatically increase the number of adherent N. gonorrhoeae quantified per assay while improving repeatability and removing observer bias. …
A flow cytometry based method, using the cell-permeable fluorescent dye 5'-carboxyfluoroscein succidyl ester (CFSE), was developed to …
Children's residential exposure to organophosphate ester flame retardants and plasticizers: Investigating exposure pathways in the TESIE study.
Phillips AL, Hammel SC, Hoffman K, Lorenzo AM, Chen A, Webster TF, Stapleton HM. Phillips AL, et al. Environ Int. 2018 Jul;116:176-185. doi: 10.1016/j.envint.2018.04.013. Epub 2018 Apr 21. Environ Int. 2018. PMID: 29689464 Free PMC article.
BACKGROUND: Following the phase-out of polybrominated diphenyl ethers (PBDEs), organophosphate esters (OPEs) have been increasingly used in consumer products and building materials for their flame retardant and plasticizing properties. ...
BACKGROUND: Following the phase-out of polybrominated diphenyl ethers (PBDEs), organophosphate esters (OPEs) have been increasingly u …
Chemical uptake into silicone wristbands over a five day period.
Samon SM, Hoffman K, Herkert N, Stapleton HM. Samon SM, et al. Environ Pollut. 2024 May 15;349:123877. doi: 10.1016/j.envpol.2024.123877. Epub 2024 Apr 2. Environ Pollut. 2024. PMID: 38574945
This study evaluated the chemical uptake rates of 113 organic pollutants from several chemical classes (i.e., polychlorinated biphenyls (PCB), organophosphate esters (OPEs), alkyl OPEs, polybrominated diphenyl ethers (PBDEs), brominated flame retardants (BFR), phthalates, …
This study evaluated the chemical uptake rates of 113 organic pollutants from several chemical classes (i.e., polychlorinated biphenyls (PCB …
Biomarkers of exposure to SVOCs in children and their demographic associations: The TESIE Study.
Hoffman K, Hammel SC, Phillips AL, Lorenzo AM, Chen A, Calafat AM, Ye X, Webster TF, Stapleton HM. Hoffman K, et al. Environ Int. 2018 Oct;119:26-36. doi: 10.1016/j.envint.2018.06.007. Epub 2018 Jun 18. Environ Int. 2018. PMID: 29929048 Free PMC article.
We enrolled 203 participants in the Toddlers Exposure to SVOCs in Indoor Environments (TESIE) study (181 provided urine samples and 90 provided serum samples).We quantified 44 biomarkers of exposure to phthalates, organophosphate esters (OPEs), parabens, phenols, antibacte …
We enrolled 203 participants in the Toddlers Exposure to SVOCs in Indoor Environments (TESIE) study (181 provided urine samples and 90 provi …
Characterizing firefighter's exposure to over 130 SVOCs using silicone wristbands: A pilot study comparing on-duty and off-duty exposures.
Levasseur JL, Hoffman K, Herkert NJ, Cooper E, Hay D, Stapleton HM. Levasseur JL, et al. Sci Total Environ. 2022 Aug 15;834:155237. doi: 10.1016/j.scitotenv.2022.155237. Epub 2022 Apr 18. Sci Total Environ. 2022. PMID: 35447169 Free PMC article.
Of these, phthalates were generally measured at the highest concentrations, followed by brominated flame retardants (BFRs) and organophosphate esters (OPEs). PFAS were measured at lower concentrations overall, but firefighter PFOS exposures while on-duty and responding to …
Of these, phthalates were generally measured at the highest concentrations, followed by brominated flame retardants (BFRs) and organophospha …
Use of flow cytometry and PCR analysis to detect 5-carboxyfluoroscein-stained obligate intracellular bacteria Lawsonia intracellularis invasion of McCoy cells.
Obradovic M, Pasternak JA, Ng SH, Wilson HL. Obradovic M, et al. J Microbiol Methods. 2016 Jul;126:60-6. doi: 10.1016/j.mimet.2016.04.015. Epub 2016 May 3. J Microbiol Methods. 2016. PMID: 27154728
In immunological research, the cell-permeable fluorescent dye 5'-carboxyfluoroscein succidyl ester (CFSE) is commonly used to quantify eukaryotic cellular proliferation. ...
In immunological research, the cell-permeable fluorescent dye 5'-carboxyfluoroscein succidyl ester (CFSE) is commonly used to quantif …
Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins.
Moheimani F, Tan JT, Brown BE, Heather AK, van Reyk DM, Davies MJ. Moheimani F, et al. Exp Diabetes Res. 2011;2011:851280. doi: 10.1155/2011/851280. Epub 2011 Aug 28. Exp Diabetes Res. 2011. PMID: 21904540 Free PMC article.
During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. ...SR-BI and CD36 prot …
During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-lik …
Regional comparison of organophosphate flame retardant (PFR) urinary metabolites and tetrabromobenzoic acid (TBBA) in mother-toddler pairs from California and New Jersey.
Butt CM, Hoffman K, Chen A, Lorenzo A, Congleton J, Stapleton HM. Butt CM, et al. Environ Int. 2016 Sep;94:627-634. doi: 10.1016/j.envint.2016.06.029. Epub 2016 Jul 7. Environ Int. 2016. PMID: 27397928 Free PMC article.
Today, the most commonly detected alternatives in residential furniture include the organophosphate flame retardants (PFRs) and the Firemaster (R) 550 mixture (FM550). Urinary levels of dialkyl and diaryl phosphate esters, and 2-ethylhexyl tetrabromobenzoate (EH-TBB) have …
Today, the most commonly detected alternatives in residential furniture include the organophosphate flame retardants (PFRs) and the Firemast …
Near infrared spectroscopy as a rapid tool to measure volatile aroma compounds in Riesling wine: possibilities and limits.
Smyth HE, Cozzolino D, Cynkar WU, Dambergs RG, Sefton M, Gishen M. Smyth HE, et al. Anal Bioanal Chem. 2008 Apr;390(7):1911-6. doi: 10.1007/s00216-008-1940-0. Epub 2008 Feb 19. Anal Bioanal Chem. 2008. PMID: 18283438
Coefficients of determination in cross validation (R(2)) and the standard error in cross validation (SECV) were 0.74 (SECV: 313.6 microg L(-1)) for esters, 0.90 (SECV: 20.9 microg L(-1)) for monoterpenes and 0.80 (SECV: 1658 microg L(-1)) for short-chain fatty acids. ...
Coefficients of determination in cross validation (R(2)) and the standard error in cross validation (SECV) were 0.74 (SECV: 313.6 microg L(- …
11 results