Mixtures of trifluoroethanol or hexafluoroisopropanol and dimethylformamide are not of general applicability for peptide condensations catalyzed by trypsin.
Bemquerer MP, Liria CW, Kitagawa K, Miranda MT, Tominaga M.
Bemquerer MP, et al. Among authors: liria cw.
J Pept Res. 1998 Jan;51(1):29-37. doi: 10.1111/j.1399-3011.1998.tb00413.x.
J Pept Res. 1998.
PMID: 9495588
Mixtures of a good hydrogen bond donor, 2,2,2-trifluoroethanol (TFE) or 1,1,1,3,3,3-hexafluroisopropanol, and an acceptor, dimethylformamide (DMF) (1:1, v/v), containing 4% buffer have been described as adequate solvent systems for trypsin-catalyzed peptide fragment condensations …
Mixtures of a good hydrogen bond donor, 2,2,2-trifluoroethanol (TFE) or 1,1,1,3,3,3-hexafluroisopropanol, and an acceptor, dimethylformamide …