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Isolation of Highly Migratory and Invasive Cells in Three-Dimensional Gels.
Takino T, Suzuki T, Seiki M. Takino T, et al. Curr Protoc Cell Biol. 2020 Mar;86(1):e103. doi: 10.1002/cpcb.103. Curr Protoc Cell Biol. 2020. PMID: 32022994
We developed a modified invasion assay in three-dimensional (3D) gels that permits isolation of invading cells as living cells, termed an invading cell-trapping (iCT) assay. A small cell strainer consisting of nylon mesh with pores of 40-m square is used in this assay. ... …
We developed a modified invasion assay in three-dimensional (3D) gels that permits isolation of invading cells as living cells, termed an in …
Using Flow Cytometry to Isolate Maize Meiocytes for Next Generation Sequencing: A Time and Labor Efficient Method.
Chang P, Tseng YF, Chen PY, Wang CR. Chang P, et al. Curr Protoc Plant Biol. 2018 Jun;3(2):e20068. doi: 10.1002/cppb.20068. Epub 2018 May 24. Curr Protoc Plant Biol. 2018. PMID: 29927118
The meiocyte fraction is enriched by size separation with cell strainers and further purified by flow cytometry. From 400 anthers, we can isolate 20,000 meiocytes at 98% purity in 6 to 8 hours. 2018 by John Wiley & Sons, Inc....
The meiocyte fraction is enriched by size separation with cell strainers and further purified by flow cytometry. From 400 anthers, we …