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Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-single-strand-conformation polymorphism.
Lee DH, Zo YG, Kim SJ. Lee DH, et al. Appl Environ Microbiol. 1996 Sep;62(9):3112-20. doi: 10.1128/aem.62.9.3112-3120.1996. Appl Environ Microbiol. 1996. PMID: 8795197 Free PMC article.
A pair of eubacterial universal primers for amplification of the variable V3 region were designed from the 16S rRNA sequences of 1,262 bacterial strains. The PCR conditions were optimized by using genomic DNAs from five gram-positive and seven gram-negative strains. ...
A pair of eubacterial universal primers for amplification of the variable V3 region were designed from the 16S rRNA sequences of 1,262 …