Mutagenesis of the uncoupling protein of brown adipose tissue. Neutralization Of E190 largely abolishes pH control of nucleotide binding.
Echtay KS, Bienengraeber M, Klingenberg M.
Echtay KS, et al. Among authors: klingenberg m.
Biochemistry. 1997 Jul 8;36(27):8253-60. doi: 10.1021/bi970513r.
Biochemistry. 1997.
PMID: 9204870
Woodward reagent K (WRK) known to interact specifically with E190 [Winkler, E., Wachter, E., and Klingenberg, M. (1997) Biochemistry 36, 148-155] abolished [14C]GTP and dansyl-GTP binding to wt UCP, whereas binding to E190Q was fully resistant to WRK. ...
Woodward reagent K (WRK) known to interact specifically with E190 [Winkler, E., Wachter, E., and Klingenberg, M. (1997) Bioche …