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A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.
Tálas A, Kulcsár PI, Weinhardt N, Borsy A, Tóth E, Szebényi K, Krausz SL, Huszár K, Vida I, Sturm Á, Gordos B, Hoffmann OI, Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E. Tálas A, et al. Among authors: kulcsar pi. DNA Res. 2017 Dec 1;24(6):609-621. doi: 10.1093/dnares/dsx029. DNA Res. 2017. PMID: 28679166 Free PMC article.
Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Tóth E, Czene BC, Kulcsár PI, Krausz SL, Tálas A, Nyeste A, Varga É, Huszár K, Weinhardt N, Ligeti Z, Borsy AÉ, Fodor E, Welker E. Tóth E, et al. Among authors: kulcsar pi. Nucleic Acids Res. 2018 Nov 2;46(19):10272-10285. doi: 10.1093/nar/gky815. Nucleic Acids Res. 2018. PMID: 30239882 Free PMC article.
Identification of New Interactions between Endolysosomal Tethering Factors.
Simon-Vecsei Z, Sőth Á, Lőrincz P, Rubics A, Tálas A, Kulcsár PI, Juhász G. Simon-Vecsei Z, et al. Among authors: kulcsar pi. J Mol Biol. 2021 Jun 25;433(13):166965. doi: 10.1016/j.jmb.2021.166965. Epub 2021 Mar 27. J Mol Biol. 2021. PMID: 33781757 Free article.
27 results