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The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast). Comparison between the Y254F and Y254L mutant proteins.
Gondry M, Dubois J, Terrier M, Lederer F. Gondry M, et al. Among authors: lederer f. Eur J Biochem. 2001 Sep;268(18):4918-27. doi: 10.1046/j.0014-2956.2001.02424.x. Eur J Biochem. 2001. PMID: 11559361 Free article.
Studies carried out with the Y254F mutant [Dubois, J., Chapman, S.K., Mathews, F.S., Reid, G.A. & Lederer, F. (1990) Biochemistry 29, 6393-6400] showed that Tyr254 does not act as a base but stabilizes the transition state. ...
Studies carried out with the Y254F mutant [Dubois, J., Chapman, S.K., Mathews, F.S., Reid, G.A. & Lederer, F. (1990 …
Substitution of Tyr254 with Phe at the active site of flavocytochrome b2: consequences on catalysis of lactate dehydrogenation.
Dubois J, Chapman SK, Mathews FS, Reid GA, Lederer F. Dubois J, et al. Among authors: lederer f. Biochemistry. 1990 Jul 10;29(27):6393-400. doi: 10.1021/bi00479a008. Biochemistry. 1990. PMID: 2207080
A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F
A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-s …
Molecular interpretation of inhibition by excess substrate in flavocytochrome b2: a study with wild-type and Y143F mutant enzymes.
Rouvière N, Mayer M, Tegoni M, Capeillère-Blandin C, Lederer F. Rouvière N, et al. Among authors: lederer f. Biochemistry. 1997 Jun 10;36(23):7126-35. doi: 10.1021/bi963035d. Biochemistry. 1997. PMID: 9188712
Previous work on the Y143F mutant enzyme provided support for these hypotheses [Miles, C. S., Rouviere-Fourmy, N., Lederer, F., Mathews, F. S., Reid, G. A., Black, M. T., & Chapman, S. ...J. 285, 187-192; Rouviere-Fourmy, N., Capeillere-Blandin, C., & …
Previous work on the Y143F mutant enzyme provided support for these hypotheses [Miles, C. S., Rouviere-Fourmy, N., Lederer, F. …
392 results