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Showing results for elisa roan
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CD8 T Cell Virus Inhibition Assay Protocol.
Xu Y, Weideman AM, Abad-Fernandez M, Mollan KR, Kallon S, Samir S, Warren JA, Clutton G, Roan N, Adimora AA, Archin N, Kuruc J, Gay C, Hudgens MG, Goonetilleke N. Xu Y, et al. Bio Protoc. 2022 Mar 20;12(6):e4354. doi: 10.21769/BioProtoc.4354. eCollection 2022 Mar 20. Bio Protoc. 2022. PMID: 35434196 Free PMC article.
The human immunodeficiency virus (HIV)-1 viral inhibition assay (VIA) measures CD8(+) T cell-mediated inhibition of HIV replication in CD4(+) T cells and is increasingly used for clinical testing of HIV vaccines and immunotherapies. ...We identify methodological and analys …
The human immunodeficiency virus (HIV)-1 viral inhibition assay (VIA) measures CD8(+) T cell-mediated inhibition of HIV replication i …
Pan HDACi Valproic Acid and Trichostatin A Show Apparently Contrasting Inflammatory Responses in Cultured J774A.1 Macrophages.
Ubah UDB, Triyasakorn K, Roan B, Conlin M, Lai JCK, Awale PS. Ubah UDB, et al. Epigenomes. 2022 Nov 3;6(4):38. doi: 10.3390/epigenomes6040038. Epigenomes. 2022. PMID: 36412793 Free PMC article.
J774A.1 macrophages were treated with either VPA or TSA for 1 h (acute treatment), followed by maximal stimulation with LPS + IFNgamma for 24 h. ELISA was used to measure the levels of proinflammatory cytokines TNFalpha, NO and IL-1beta from the culture medium. ...
J774A.1 macrophages were treated with either VPA or TSA for 1 h (acute treatment), followed by maximal stimulation with LPS + IFNgamma for 2 …
Loss of Preexisting Immunological Memory Among Human Immunodeficiency Virus-Infected Women Despite Immune Reconstitution With Antiretroviral Therapy.
Thomas A, Hammarlund E, Gao L, Holman S, Michel KG, Glesby M, Villacres MC, Golub ET, Roan NR, French AL, Augenbraun MH, Slifka MK. Thomas A, et al. J Infect Dis. 2020 Jun 29;222(2):243-251. doi: 10.1093/infdis/jiz678. J Infect Dis. 2020. PMID: 31867597 Free PMC article.
Total memory T-cell responses were measured after anti-CD3 or vaccinia virus (VV) stimulation to measure T cells elicited after childhood smallpox vaccination. VV-specific antibodies were measured by means of enzyme-linked immunosorbent assay (ELISA …
Total memory T-cell responses were measured after anti-CD3 or vaccinia virus (VV) stimulation to measure T cells elicited after childhood sm …
TREK-1 Regulates Cytokine Secretion from Cultured Human Alveolar Epithelial Cells Independently of Cytoskeletal Rearrangements.
Schwingshackl A, Roan E, Teng B, Waters CM. Schwingshackl A, et al. PLoS One. 2015 May 22;10(5):e0126781. doi: 10.1371/journal.pone.0126781. eCollection 2015. PLoS One. 2015. PMID: 26001192 Free PMC article.
METHODS: We determined F-actin and alpha-tubulin contents of control, TREK-1-deficient and TREK-1-overexpressing human A549 cells by confocal microscopy and western blotting, and measured IL-6 and MCP-1 levels using real-time PCR and ELISA. RESULTS: Cytochalasin D decrease …
METHODS: We determined F-actin and alpha-tubulin contents of control, TREK-1-deficient and TREK-1-overexpressing human A549 cells by confoca …
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