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Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods.
Sorzano COS, Jiménez-Moreno A, Maluenda D, Ramírez-Aportela E, Martínez M, Cuervo A, Melero R, Conesa JJ, Sánchez-García R, Strelak D, Filipovic J, Fernández-Giménez E, de Isidro-Gómez F, Herreros D, Conesa P, Del Caño L, Fonseca Y, de la Morena JJ, Macías JR, Losana P, Marabini R, Carazo JM. Sorzano COS, et al. Among authors: melero r. Methods Mol Biol. 2021;2305:257-289. doi: 10.1007/978-1-0716-1406-8_13. Methods Mol Biol. 2021. PMID: 33950394
Blind estimation of DED camera gain in Electron Microscopy.
Sorzano COS, Fernández-Giménez E, Peredo-Robinson V, Vargas J, Majtner T, Caffarena G, Otón J, Vilas JL, de la Rosa-Trevín JM, Melero R, Gómez-Blanco J, Cuenca J, Del Cano L, Conesa P, Marabini R, Carazo JM. Sorzano COS, et al. Among authors: melero r. J Struct Biol. 2018 Aug;203(2):90-93. doi: 10.1016/j.jsb.2018.03.007. Epub 2018 Mar 15. J Struct Biol. 2018. PMID: 29551714
Using Scipion for stream image processing at Cryo-EM facilities.
Gómez-Blanco J, de la Rosa-Trevín JM, Marabini R, Del Cano L, Jiménez A, Martínez M, Melero R, Majtner T, Maluenda D, Mota J, Rancel Y, Ramírez-Aportela E, Vilas JL, Carroni M, Fleischmann S, Lindahl E, Ashton AW, Basham M, Clare DK, Savage K, Siebert CA, Sharov GG, Sorzano COS, Conesa P, Carazo JM. Gómez-Blanco J, et al. Among authors: melero r. J Struct Biol. 2018 Dec;204(3):457-463. doi: 10.1016/j.jsb.2018.10.001. Epub 2018 Oct 5. J Struct Biol. 2018. PMID: 30296492 Free PMC article.
Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy.
Sorzano COS, Jiménez A, Mota J, Vilas JL, Maluenda D, Martínez M, Ramírez-Aportela E, Majtner T, Segura J, Sánchez-García R, Rancel Y, Del Caño L, Conesa P, Melero R, Jonic S, Vargas J, Cazals F, Freyberg Z, Krieger J, Bahar I, Marabini R, Carazo JM. Sorzano COS, et al. Among authors: melero r. Acta Crystallogr F Struct Biol Commun. 2019 Jan 1;75(Pt 1):19-32. doi: 10.1107/S2053230X18015108. Epub 2019 Jan 1. Acta Crystallogr F Struct Biol Commun. 2019. PMID: 30605122 Free PMC article. Review.
Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion.
Maluenda D, Majtner T, Horvath P, Vilas JL, Jiménez-Moreno A, Mota J, Ramírez-Aportela E, Sánchez-García R, Conesa P, Del Caño L, Rancel Y, Fonseca Y, Martínez M, Sharov G, García CA, Strelak D, Melero R, Marabini R, Carazo JM, Sorzano COS. Maluenda D, et al. Among authors: melero r. Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):882-894. doi: 10.1107/S2059798319011860. Epub 2019 Oct 1. Acta Crystallogr D Struct Biol. 2019. PMID: 31588920 Free PMC article.
Integration of Cryo-EM Model Building Software in Scipion.
Martínez M, Jiménez-Moreno A, Maluenda D, Ramírez-Aportela E, Melero R, Cuervo A, Conesa P, Del Caño L, Fonseca YC, Sánchez-García R, Strelak D, Conesa JJ, Fernández-Giménez E, de Isidro F, Sorzano COS, Carazo JM, Marabini R. Martínez M, et al. Among authors: melero r. J Chem Inf Model. 2020 May 26;60(5):2533-2540. doi: 10.1021/acs.jcim.9b01032. Epub 2020 Feb 11. J Chem Inf Model. 2020. PMID: 31994878 Free article.
Algorithmic robustness to preferred orientations in single particle analysis by CryoEM.
Sorzano COS, Semchonok D, Lin SC, Lo YC, Vilas JL, Jiménez-Moreno A, Gragera M, Vacca S, Maluenda D, Martínez M, Ramírez-Aportela E, Melero R, Cuervo A, Conesa JJ, Conesa P, Losana P, Caño LD, de la Morena JJ, Fonseca YC, Sánchez-García R, Strelak D, Fernández-Giménez E, de Isidro F, Herreros D, Kastritis PL, Marabini R, Bruce BD, Carazo JM. Sorzano COS, et al. Among authors: melero r. J Struct Biol. 2021 Mar;213(1):107695. doi: 10.1016/j.jsb.2020.107695. Epub 2021 Jan 7. J Struct Biol. 2021. PMID: 33421545 Free article.
Cryo-EM and Single-Particle Analysis with Scipion.
Jiménez-Moreno A, Del Caño L, Martínez M, Ramírez-Aportela E, Cuervo A, Melero R, Sánchez-García R, Strelak D, Fernández-Giménez E, de Isidro-Gómez FP, Herreros D, Conesa P, Fonseca Y, Maluenda D, Jiménez de la Morena J, Macías JR, Losana P, Marabini R, Carazo JM, Sorzano COS. Jiménez-Moreno A, et al. Among authors: melero r. J Vis Exp. 2021 May 29;(171). doi: 10.3791/62261. J Vis Exp. 2021. PMID: 34125107
99 results