HLA class I sequence-based typing using DNA recovered from frozen plasma.
Cotton LA, Abdur Rahman M, Ng C, Le AQ, Milloy MJ, Mo T, Brumme ZL.
Cotton LA, et al. Among authors: ng c.
J Immunol Methods. 2012 Aug 31;382(1-2):40-7. doi: 10.1016/j.jim.2012.05.003. Epub 2012 May 11.
J Immunol Methods. 2012.
PMID: 22584155
Extractions yielded median [IQR] nucleic acid concentrations of 85.7 [47.0-130.0]ng/mul and 2.2 [1.7-2.6]ng/mul from PBMC and plasma, respectively. Following extraction, ~1000 base pair regions spanning exons 2 and 3 of HLA-A, -B and -C were amplified indepen …
Extractions yielded median [IQR] nucleic acid concentrations of 85.7 [47.0-130.0]ng/mul and 2.2 [1.7-2.6]ng/mul from PBMC and …