Single channel recording of a mitochondrial calcium uniporter.
Wu G, Li S, Zong G, Liu X, Fei S, Shen L, Guan X, Yang X, Shen Y.
Wu G, et al.
Biochem Biophys Res Commun. 2018 Jan 29;496(1):127-132. doi: 10.1016/j.bbrc.2018.01.010. Epub 2018 Jan 4.
Biochem Biophys Res Commun. 2018.
PMID: 29307826
Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. We showed that MCU alone from Pyronema omphalodes (pMCU) is active with prominent single channel Ca(2+) currents. ...
Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. …