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Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange.
Labrijn AF, Meesters JI, de Goeij BE, van den Bremer ET, Neijssen J, van Kampen MD, Strumane K, Verploegen S, Kundu A, Gramer MJ, van Berkel PH, van de Winkel JG, Schuurman J, Parren PW. Labrijn AF, et al. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5145-50. doi: 10.1073/pnas.1220145110. Epub 2013 Mar 11. Proc Natl Acad Sci U S A. 2013. PMID: 23479652 Free PMC article.
Species-specific determinants in the IgG CH3 domain enable Fab-arm exchange by affecting the noncovalent CH3-CH3 interaction strength.
Labrijn AF, Rispens T, Meesters J, Rose RJ, den Bleker TH, Loverix S, van den Bremer ET, Neijssen J, Vink T, Lasters I, Aalberse RC, Heck AJ, van de Winkel JG, Schuurman J, Parren PW. Labrijn AF, et al. J Immunol. 2011 Sep 15;187(6):3238-46. doi: 10.4049/jimmunol.1003336. Epub 2011 Aug 12. J Immunol. 2011. PMID: 21841137
Cysteine-SILAC Mass Spectrometry Enabling the Identification and Quantitation of Scrambled Interchain Disulfide Bonds: Preservation of Native Heavy-Light Chain Pairing in Bispecific IgGs Generated by Controlled Fab-arm Exchange.
van den Bremer ETJ, Labrijn AF, van den Boogaard R, Priem P, Scheffler K, Melis JPM, Schuurman J, Parren PWHI, de Jong RN. van den Bremer ETJ, et al. Among authors: parren pwhi. Anal Chem. 2017 Oct 17;89(20):10873-10882. doi: 10.1021/acs.analchem.7b02543. Epub 2017 Oct 2. Anal Chem. 2017. PMID: 28922593
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