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Conformational stability changes of the amino terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system produced by substituting alanine or glutamate for the active-site histidine 189: implications for phosphorylation effects.
Ginsburg A, Szczepanowski RH, Ruvinov SB, Nosworthy NJ, Sondej M, Umland TC, Peterkofsky A. Ginsburg A, et al. Among authors: peterkofsky a. Protein Sci. 2000 Jun;9(6):1085-94. doi: 10.1110/ps.9.6.1085. Protein Sci. 2000. PMID: 10892802 Free PMC article.
Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR.
Garrett DS, Seok YJ, Liao DI, Peterkofsky A, Gronenborn AM, Clore GM. Garrett DS, et al. Among authors: peterkofsky a. Biochemistry. 1997 Mar 4;36(9):2517-30. doi: 10.1021/bi962924y. Biochemistry. 1997. PMID: 9054557
The active site His189 is located in a cleft at the junction of the alpha and alpha/beta domains and has a pKa of approximately 6.3. His189 has a trans conformation about chi1, a g+ conformation about chi2, and its Nepsilon2 atom accepts a hydro …
The active site His189 is located in a cleft at the junction of the alpha and alpha/beta domains and has a pKa of approximatel …
Identification by NMR of the binding surface for the histidine-containing phosphocarrier protein HPr on the N-terminal domain of enzyme I of the Escherichia coli phosphotransferase system.
Garrett DS, Seok YJ, Peterkofsky A, Clore GM, Gronenborn AM. Garrett DS, et al. Among authors: peterkofsky a. Biochemistry. 1997 Apr 15;36(15):4393-8. doi: 10.1021/bi970221q. Biochemistry. 1997. PMID: 9109646
By mapping the backbone chemical shift perturbations on the three-dimensional solution structure of EIN [Garrett, D. S., Seok, Y.-J., Liao, D.-I., Peterkofsky, A., Gronenborn, A. M., & Clore, G. M. (1997) Biochemistry 36, 2517-2530], we have identified th …
By mapping the backbone chemical shift perturbations on the three-dimensional solution structure of EIN [Garrett, D. S., Seok, Y.-J., Liao, …
153 results