Chemical Proteomics Reveals Off-Targets of the Anandamide Reuptake Inhibitor WOBE437.
Gagestein B, Stevens AF, Fazio D, Florea BI, van der Wel T, Bakker AT, Fezza F, Dulk HD, Overkleeft HS, Maccarrone M, van der Stelt M.
Gagestein B, et al.
ACS Chem Biol. 2022 May 20;17(5):1174-1183. doi: 10.1021/acschembio.2c00122. Epub 2022 Apr 28.
ACS Chem Biol. 2022.
PMID: 35482948
Free PMC article.
Using pac-WOBE, we identified saccharopine dehydrogenase-like oxidoreductase (SCCPDH), vesicle amine transport 1 (VAT1), and ferrochelatase (FECH) as WOBE437-interacting proteins in Neuro-2a cells. Further genetic studies indicated that SCCPDH and VAT1 were not resp …
Using pac-WOBE, we identified saccharopine dehydrogenase-like oxidoreductase (SCCPDH), vesicle amine transport 1 (VAT1), and ferroche …