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UbaLAI is a monomeric Type IIE restriction enzyme.
Sasnauskas G, Tamulaitiene G, Tamulaitis G, Calyševa J, Laime M, Rimšeliene R, Lubys A, Siksnys V. Sasnauskas G, et al. Among authors: siksnys v. Nucleic Acids Res. 2017 Sep 19;45(16):9583-9594. doi: 10.1093/nar/gkx634. Nucleic Acids Res. 2017. PMID: 28934493 Free PMC article.
Site-directed mutagenesis of putative active site residues of MunI restriction endonuclease: replacement of catalytically essential carboxylate residues triggers DNA binding specificity.
Lagunavicius A, Siksnys V. Lagunavicius A, et al. Among authors: siksnys v. Biochemistry. 1997 Sep 16;36(37):11086-92. doi: 10.1021/bi963125i. Biochemistry. 1997. PMID: 9287151
Mapping of the conserved sequence regions in the restriction endonucleases MunI (C/AATTG) and EcoRI (G/AATTC) to the known X-ray structure of EcoRI allowed us to identify the sequence motif 82PDX14EXK as the putative catalytic/Mg2+ ion binding site of MunI [Siksnys, V …
Mapping of the conserved sequence regions in the restriction endonucleases MunI (C/AATTG) and EcoRI (G/AATTC) to the known X-ray structure o …
DNA binding specificity of MunI restriction endonuclease is controlled by pH and calcium ions: involvement of active site carboxylate residues.
Lagunavicius A, Grazulis S, Balciunaite E, Vainius D, Siksnys V. Lagunavicius A, et al. Among authors: siksnys v. Biochemistry. 1997 Sep 16;36(37):11093-9. doi: 10.1021/bi963126a. Biochemistry. 1997. PMID: 9287152
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that at pH 8.3 in the absence of Mg2+, MunI restriction endonuclease exhibits little DNA binding specificity, as compared with the D83A and E98A m …
Gel shift analysis reveals [Lagunavicius, A., & Siksnys, V. (1997) Biochemistry 36 (preceding paper in this issue)] that a …
Plasmid DNA cleavage by MunI restriction enzyme: single-turnover and steady-state kinetic analysis.
Sasnauskas G, Jeltsch A, Pingoud A, Siksnys V. Sasnauskas G, et al. Among authors: siksnys v. Biochemistry. 1999 Mar 30;38(13):4028-36. doi: 10.1021/bi982456n. Biochemistry. 1999. PMID: 10194315
In the absence of metal ions, protonation of an ionizable residue with a pKa > 7.0, most likely one of the active site carboxylates, controls the DNA binding specificity of MunI [Lagunavicius, A., Grazulis, S., Balciunaite, E., Vainius, D., and Siksnys, V. (1997) …
In the absence of metal ions, protonation of an ionizable residue with a pKa > 7.0, most likely one of the active site carboxylates, cont …
The Cfr10I restriction enzyme is functional as a tetramer.
Siksnys V, Skirgaila R, Sasnauskas G, Urbanke C, Cherny D, Grazulis S, Huber R. Siksnys V, et al. J Mol Biol. 1999 Sep 3;291(5):1105-18. doi: 10.1006/jmbi.1999.2977. J Mol Biol. 1999. PMID: 10518946
133 results