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Mechanisms of translational regulation by a human eIF5-mimic protein.
Singh CR, Watanabe R, Zhou D, Jennings MD, Fukao A, Lee B, Ikeda Y, Chiorini JA, Campbell SG, Ashe MP, Fujiwara T, Wek RC, Pavitt GD, Asano K. Singh CR, et al. Nucleic Acids Res. 2011 Oct;39(19):8314-28. doi: 10.1093/nar/gkr339. Epub 2011 Jul 10. Nucleic Acids Res. 2011. PMID: 21745818 Free PMC article.
Yeast phenotypic assays on translational control.
Lee B, Udagawa T, Singh CR, Asano K. Lee B, et al. Among authors: singh cr. Methods Enzymol. 2007;429:105-37. doi: 10.1016/S0076-6879(07)29006-8. Methods Enzymol. 2007. PMID: 17913621
Sequential eukaryotic translation initiation factor 5 (eIF5) binding to the charged disordered segments of eIF4G and eIF2β stabilizes the 48S preinitiation complex and promotes its shift to the initiation mode.
Singh CR, Watanabe R, Chowdhury W, Hiraishi H, Murai MJ, Yamamoto Y, Miles D, Ikeda Y, Asano M, Asano K. Singh CR, et al. Mol Cell Biol. 2012 Oct;32(19):3978-89. doi: 10.1128/MCB.00376-12. Epub 2012 Jul 30. Mol Cell Biol. 2012. PMID: 22851688 Free PMC article.
Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.
Kozel C, Thompson B, Hustak S, Moore C, Nakashima A, Singh CR, Reid M, Cox C, Papadopoulos E, Luna RE, Anderson A, Tagami H, Hiraishi H, Slone EA, Yoshino KI, Asano M, Gillaspie S, Nietfeld J, Perchellet JP, Rothenburg S, Masai H, Wagner G, Beeser A, Kikkawa U, Fleming SD, Asano K. Kozel C, et al. Among authors: singh cr. Nucleic Acids Res. 2016 Oct 14;44(18):8704-8713. doi: 10.1093/nar/gkw559. Epub 2016 Jun 20. Nucleic Acids Res. 2016. PMID: 27325740 Free PMC article.
Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.
Tang L, Morris J, Wan J, Moore C, Fujita Y, Gillaspie S, Aube E, Nanda J, Marques M, Jangal M, Anderson A, Cox C, Hiraishi H, Dong L, Saito H, Singh CR, Witcher M, Topisirovic I, Qian SB, Asano K. Tang L, et al. Among authors: singh cr. Nucleic Acids Res. 2017 Nov 16;45(20):11941-11953. doi: 10.1093/nar/gkx808. Nucleic Acids Res. 2017. PMID: 28981728 Free PMC article.
52 results