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Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.
Sequeira AF, Turchetto J, Saez NJ, Peysson F, Ramond L, Duhoo Y, Blémont M, Fernandes VO, Gama LT, Ferreira LM, Guerreiro CI, Gilles N, Darbon H, Fontes CM, Vincentelli R. Sequeira AF, et al. Among authors: vincentelli r. Microb Cell Fact. 2017 Jan 17;16(1):4. doi: 10.1186/s12934-016-0618-0. Microb Cell Fact. 2017. PMID: 28093085 Free PMC article.
High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.
Turchetto J, Sequeira AF, Ramond L, Peysson F, Brás JL, Saez NJ, Duhoo Y, Blémont M, Guerreiro CI, Quinton L, De Pauw E, Gilles N, Darbon H, Fontes CM, Vincentelli R. Turchetto J, et al. Among authors: vincentelli r. Microb Cell Fact. 2017 Jan 17;16(1):6. doi: 10.1186/s12934-016-0617-1. Microb Cell Fact. 2017. PMID: 28095880 Free PMC article.
97 results