Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2005 1
2007 1
2008 2
2009 1
2010 1
2011 3
2012 2
2013 5
2014 5
2015 3
2018 8
2019 4
2020 5
2021 3
2022 2
2023 5
2024 0

Text availability

Article attribute

Article type

Publication date

Search Results

45 results

Results by year

Filters applied: . Clear all
Page 1
Showing results for amplicon vl
Your search for ampliron-vl retrieved no results
Amplicon-based next-generation sequencing reveals the co-existence of multiple Leishmania species in patients with visceral leishmaniasis.
Castillo-Castañeda A, Patiño LH, Muñoz M, Ayala MS, Segura M, Bautista J, Shaban MV, Paniz-Mondolfi A, Ramírez JD. Castillo-Castañeda A, et al. Int J Infect Dis. 2022 Feb;115:35-38. doi: 10.1016/j.ijid.2021.11.029. Epub 2021 Dec 1. Int J Infect Dis. 2022. PMID: 34863923 Free article.
This study used an amplicon-based next-generation sequencing approach to identify VL aetiologic species using high-depth sequencing targeting a region on the Heat Shock Protein 70 gene. In this first approach, six samples from five patients diagnosed with VL …
This study used an amplicon-based next-generation sequencing approach to identify VL aetiologic species using high-depth seque …
Genetics, Transcriptomics and Meta-Taxonomics in Visceral Leishmaniasis.
Blackwell JM, Fakiola M, Singh OP. Blackwell JM, et al. Front Cell Infect Microbiol. 2020 Nov 25;10:590888. doi: 10.3389/fcimb.2020.590888. eCollection 2020. Front Cell Infect Microbiol. 2020. PMID: 33324576 Free PMC article. Review.
Visceral leishmaniasis (VL) caused by parasites of the Leishmania donovani complex can be fatal in susceptible individuals. ...Here we review results from studies using three omics-based approaches to study VL caused by L. donovani in India: (i) chip-based analysis …
Visceral leishmaniasis (VL) caused by parasites of the Leishmania donovani complex can be fatal in susceptible individuals. ...Here w …
Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering.
Lowden MJ, Lei EK, Hussack G, Henry KA. Lowden MJ, et al. Methods Mol Biol. 2023;2702:489-540. doi: 10.1007/978-1-0716-3381-6_26. Methods Mol Biol. 2023. PMID: 37679637
In this chapter, we provide simple and rapid protocols for producing and sequencing multiplexed immunoglobulin variable domain (V(H)H, V(H,) or V(L)) amplicons derived from a variety of sources using the Illumina MiSeq platform. Generation of such amplicon libraries …
In this chapter, we provide simple and rapid protocols for producing and sequencing multiplexed immunoglobulin variable domain (V(H)H, V(H,) …
First report of cutaneous leishmaniasis caused by Leishmania donovani in Ethiopia.
Amare GA, Mekonnen GG, Kassa M, Addisu A, Kendie DA, Tegegne B, Abera A, Tadesse D, Getahun S, Wondmagegn YM, Merdekios B, Asres MS, van Griensven J, Van der Auwera G, van Henten S, Pareyn M. Amare GA, et al. Parasit Vectors. 2023 Dec 16;16(1):457. doi: 10.1186/s13071-023-06057-9. Parasit Vectors. 2023. PMID: 38104111 Free PMC article.
BACKGROUND: Leishmaniasis is a common neglected tropical disease in Ethiopia. Visceral leishmaniasis (VL) caused by Leishmania donovani presents in the lowlands, while cutaneous leishmaniasis (CL) affects people living in the highlands. ...In four samples originating from …
BACKGROUND: Leishmaniasis is a common neglected tropical disease in Ethiopia. Visceral leishmaniasis (VL) caused by Leishmania donova …
Associations of functional human leucocyte antigen class I groups with HIV viral load in a heterogeneous cohort.
Zucco AG, Bennedbæk M, Ekenberg C, Gabrielaite M, Leung P, Polizzotto MN, Kan V, Murray DD, Lundgren JD, MacPherson CR; INSIGHT START study group. Zucco AG, et al. AIDS. 2023 Sep 1;37(11):1643-1650. doi: 10.1097/QAD.0000000000003557. Epub 2023 Apr 3. AIDS. 2023. PMID: 37534724 Free PMC article.
OBJECTIVE: Human leucocyte antigen (HLA) class I alleles are the main host genetic factors involved in controlling HIV-1 viral load (VL). Nevertheless, HLA diversity has proven a significant challenge in association studies. ...These functional HLA class I clusters were us …
OBJECTIVE: Human leucocyte antigen (HLA) class I alleles are the main host genetic factors involved in controlling HIV-1 viral load (VL …
A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries.
Vollmer L, Krah S, Zielonka S, Yanakieva D. Vollmer L, et al. Methods Mol Biol. 2023;2681:161-173. doi: 10.1007/978-1-0716-3279-6_10. Methods Mol Biol. 2023. PMID: 37405648
While in vivo antibody repertoires are matured and selected as a specific pair of variable heavy and light chains (VH and VL) with optimal specificity and affinity, during the recombinant generation of in vitro libraries, the native sequence pairing is not maintained. Here …
While in vivo antibody repertoires are matured and selected as a specific pair of variable heavy and light chains (VH and VL) with op …
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains.
Zackay A, Nasereddin A, Takele Y, Tadesse D, Hailu W, Hurissa Z, Yifru S, Weldegebreal T, Diro E, Kassahun A, Hailu A, Jaffe CL. Zackay A, et al. PLoS Negl Trop Dis. 2013;7(1):e2031. doi: 10.1371/journal.pntd.0002031. Epub 2013 Jan 24. PLoS Negl Trop Dis. 2013. PMID: 23358849 Free PMC article.
Parasites in disparate regions are transmitted by different vectors, and cluster in distinctive genotypes. Recently isolated strains from VL and HIV-VL co-infected patients in north and south Ethiopia were characterized as part of a longitudinal study on VL t …
Parasites in disparate regions are transmitted by different vectors, and cluster in distinctive genotypes. Recently isolated strains from …
Functional interrogation and mining of natively paired human VH:VL antibody repertoires.
Wang B, DeKosky BJ, Timm MR, Lee J, Normandin E, Misasi J, Kong R, McDaniel JR, Delidakis G, Leigh KE, Niezold T, Choi CW, Viox EG, Fahad A, Cagigi A, Ploquin A, Leung K, Yang ES, Kong WP, Voss WN, Schmidt AG, Moody MA, Ambrozak DR, Henry AR, Laboune F, Ledgerwood JE, Graham BS, Connors M, Douek DC, Sullivan NJ, Ellington AD, Mascola JR, Georgiou G. Wang B, et al. Nat Biotechnol. 2018 Feb;36(2):152-155. doi: 10.1038/nbt.4052. Epub 2018 Jan 8. Nat Biotechnol. 2018. PMID: 29309060 Free PMC article.
Libraries of natively paired, variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. ...
Libraries of natively paired, variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is …
Protocol for nearly full-length sequencing of HIV-1 RNA from plasma.
Nadai Y, Eyzaguirre LM, Constantine NT, Sill AM, Cleghorn F, Blattner WA, Carr JK. Nadai Y, et al. PLoS One. 2008 Jan 9;3(1):e1420. doi: 10.1371/journal.pone.0001420. PLoS One. 2008. PMID: 18183300 Free PMC article.
Two or three overlapping regions were PCR amplified to cover the entire viral genome and sequenced for verification. The success of the procedure was sensitive to VL but was routinely successful for VL greater than 10(5) and the rate declined in proportion to the …
Two or three overlapping regions were PCR amplified to cover the entire viral genome and sequenced for verification. The success of the proc …
Subtype diversity and emergence of drug resistance in HIV-1 in solapur district of Maharashtra, India.
Karad DD, Tandon R, Arya A, Sonawane KD, Chavan AS, Kharat AS. Karad DD, et al. Iran J Microbiol. 2022 Oct;14(5):730-739. doi: 10.18502/ijm.v14i5.10969. Iran J Microbiol. 2022. PMID: 36531813 Free PMC article.

MATERIALS AND METHODS: In a cross sectional study, forty two ART-experienced HIV-1-infected patients with CD4+ count < 200 cells ml-1 and viral load (VL) > 3, 000 copies ml-1 were recruited. All patients belonged to Maharashtra State of India near Barshi Solapur and

…

MATERIALS AND METHODS: In a cross sectional study, forty two ART-experienced HIV-1-infected patients with CD4+ count < 200 cells ml-1 and

…
45 results