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A real time Metridia luciferase based non-invasive reporter assay of mammalian cell viability and cytotoxicity via the β-actin promoter and enhancer.
Lupold SE, Johnson T, Chowdhury WH, Rodriguez R. Lupold SE, et al. PLoS One. 2012;7(5):e36535. doi: 10.1371/journal.pone.0036535. Epub 2012 May 9. PLoS One. 2012. PMID: 22590558 Free PMC article.
Here we have adapted the secreted bioluminescent reporter gene, Metridia luciferase, for use in a real-time viability assay for mammalian cells. The coding region of the marine copepod gene has been codon optimized for expression in human cells (hMLuc) and placed under the …
Here we have adapted the secreted bioluminescent reporter gene, Metridia luciferase, for use in a real-time viability assay for mamma …
Detection of neuroendocrine tumors using promoter-specific secreted Gaussia luciferase.
Tseng AW, Akerstrom V, Chen C, Breslin MB, Lan MS. Tseng AW, et al. Int J Oncol. 2016 Jan;48(1):173-80. doi: 10.3892/ijo.2015.3223. Epub 2015 Oct 30. Int J Oncol. 2016. PMID: 26530405
In order to retain the tumor-specificity of the INSM1 promoter, we have modified the promoter using the core insulator sequence from the chicken beta-globin HS4 insulator and the neuronal restrictive silencing element (NRSE). This modified INSM1-promoter can retain NE tumo …
In order to retain the tumor-specificity of the INSM1 promoter, we have modified the promoter using the core insulator sequence from the chi …