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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1905 1
1907 1
1909 1
1914 1
1922 1
1923 3
1924 1
1926 1
1930 1
1931 2
1932 2
1935 1
1938 1
1939 2
1940 1
1942 1
1944 2
1945 1
1948 2
1949 1
1950 3
1951 7
1952 8
1953 8
1954 12
1955 11
1956 10
1957 18
1958 23
1959 7
1960 21
1961 28
1962 28
1963 56
1964 82
1965 110
1966 129
1967 157
1968 281
1969 296
1970 335
1971 436
1972 529
1973 569
1974 720
1975 642
1976 688
1977 641
1978 637
1979 689
1980 747
1981 762
1982 757
1983 788
1984 799
1985 800
1986 776
1987 745
1988 730
1989 758
1990 609
1991 617
1992 564
1993 594
1994 589
1995 507
1996 525
1997 515
1998 519
1999 518
2000 591
2001 595
2002 569
2003 612
2004 665
2005 779
2006 798
2007 851
2008 973
2009 1040
2010 1082
2011 1112
2012 1113
2013 1038
2014 1201
2015 1205
2016 1254
2017 1187
2018 1266
2019 1328
2020 1445
2021 1432
2022 1401
2023 1308
2024 574

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42,589 results

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Page 1
Improving Cell Recovery: Freezing and Thawing Optimization of Induced Pluripotent Stem Cells.
Uhrig M, Ezquer F, Ezquer M. Uhrig M, et al. Cells. 2022 Feb 24;11(5):799. doi: 10.3390/cells11050799. Cells. 2022. PMID: 35269421 Free PMC article. Review.
Optimized freezing and thawing methods are required for good cell attachment and survival. In this review, we concentrate on these two aspects, freezing and thawing, but also discuss further factors influencing cell recovery such as cell storage …
Optimized freezing and thawing methods are required for good cell attachment and survival. In this review, we concentrate on t …
Culture of Adherent Cancer Cell Lines.
Noel V, Berry MD. Noel V, et al. Methods Mol Biol. 2022;2508:19-29. doi: 10.1007/978-1-0716-2376-3_3. Methods Mol Biol. 2022. PMID: 35737230
Due to the adherent nature of the cells, enzymes, such as trypsin, are required to lift the cells from the cell culture vessel for harvesting or subculturing. ...In this chapter, we outline the protocols for culturing and maintaining adherent cells. We …
Due to the adherent nature of the cells, enzymes, such as trypsin, are required to lift the cells from the cell culture …
Social sperm freezing.
Pennings G, Couture V, Ombelet W. Pennings G, et al. Hum Reprod. 2021 Mar 18;36(4):833-839. doi: 10.1093/humrep/deaa373. Hum Reprod. 2021. PMID: 33501976
Increased paternal age has been associated with lower fertility and higher genetic risk for the offspring. One way to prevent these consequences is to freeze sperm at a young age. Social sperm freezing could be developed in a way similar to social oocyte freezing …
Increased paternal age has been associated with lower fertility and higher genetic risk for the offspring. One way to prevent these conseque …
High-pressure freezing, cellular tomography, and structural cell biology.
McDonald KL, Auer M. McDonald KL, et al. Biotechniques. 2006 Aug;41(2):137, 139, 141 passim. doi: 10.2144/000112226. Biotechniques. 2006. PMID: 16925014 Free article. Review.
Structural cell biology, which we define as electron microscopic analysis of intact cells, suffered a loss of interest and activity following the advances in light microscopy beginning in the 1990s. ...We discuss how low temperature methods, especially high-pressure …
Structural cell biology, which we define as electron microscopic analysis of intact cells, suffered a loss of interest and act …
Non-toxic freezing media to retain the stem cell reserves in adipose tissues.
Shaik S, Wu X, Gimble JM, Devireddy R. Shaik S, et al. Cryobiology. 2020 Oct;96:137-144. doi: 10.1016/j.cryobiol.2020.07.005. Epub 2020 Jul 17. Cryobiology. 2020. PMID: 32687840
After 1 month of freezing storage, SVF cells and ASCs were isolated from the frozen-thawed lipoaspirates by digestion with collagenase type I. ...The SVF isolated from lipoaspirates frozen at -80 C retained comparable cell viability with the tested freezin …
After 1 month of freezing storage, SVF cells and ASCs were isolated from the frozen-thawed lipoaspirates by digestion with col …
Cell-cell contact affects membrane integrity after intracellular freezing.
Acker JP, McGann LE. Acker JP, et al. Cryobiology. 2000 Feb;40(1):54-63. doi: 10.1006/cryo.1999.2221. Cryobiology. 2000. PMID: 10679150
The response of cells to freezing depends critically on the presence of an intact cell membrane. ...For cells in a monolayer, with cell-cell contact, intracellular ice formation did not result in the immediate disruption of the plasma mem …
The response of cells to freezing depends critically on the presence of an intact cell membrane. ...For cells in …
Microfluidics for cryopreservation.
Zhao G, Fu J. Zhao G, et al. Biotechnol Adv. 2017 Mar-Apr;35(2):323-336. doi: 10.1016/j.biotechadv.2017.01.006. Epub 2017 Jan 30. Biotechnol Adv. 2017. PMID: 28153517 Free PMC article. Review.
Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. ...This review describes applications …
Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell …
Cryopreservation of plant cell lines.
Schumacher HM, Westphal M, Heine-Dobbernack E. Schumacher HM, et al. Methods Mol Biol. 2015;1257:423-9. doi: 10.1007/978-1-4939-2193-5_21. Methods Mol Biol. 2015. PMID: 25428021
Plant cell cultures may consist of dedifferentiated cells as well as of cells showing embryogenic potential. ...The controlled-rate slow freezing approach which is described here, however, remains to be the gold standard for cell cultures. ...
Plant cell cultures may consist of dedifferentiated cells as well as of cells showing embryogenic potential. ...The con …
A Vivens Ex Vivo Study on the Synergistic Effect of Electrolysis and Freezing on the Cell Nucleus.
Lugnani F, Zanconati F, Marcuzzo T, Bottin C, Mikus P, Guenther E, Klein N, Rubinsky L, Stehling MK, Rubinsky B. Lugnani F, et al. PLoS One. 2015 Dec 22;10(12):e0145133. doi: 10.1371/journal.pone.0145133. eCollection 2015. PLoS One. 2015. PMID: 26695185 Free PMC article.
Despite major advantages they also have some disadvantages. Cryosurgery cannot induce cell death at high subzero freezing temperatures and requires multiple freeze thaw cycles, while EChT requires high concentrations of electrolytic products-which makes it a …
Despite major advantages they also have some disadvantages. Cryosurgery cannot induce cell death at high subzero freezing temp …
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