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Enhancing the activity and succinyl-CoA specificity of 3-ketoacyl-CoA thiolase Tfu_0875 through rational binding pocket engineering.
Synth Syst Biotechnol. 2024 Apr 20;9(3):558-568. doi: 10.1016/j.synbio.2024.04.014. eCollection 2024 Sep.
Synth Syst Biotechnol. 2024.
PMID: 38694995
Free PMC article.
Finally, our rationally designed mutant, Tfu_0875(N249W/L163H/E217L), increased the adipic acid titer by 1.35-fold compared to the wild type Tfu_0875 in shake flask. The demonstrated enzymatic methods provide a promising enzyme variant for the adipic acid production. ...
Finally, our rationally designed mutant, Tfu_0875(N249W/L163H/E217L), increased the adipic acid titer by 1.35-fold compared to the wild type …
Synthesis of tertiary alpha-hydroxy acids by silylene transfer to alpha-keto esters.
Howard BE, Woerpel KA.
Howard BE, et al.
Org Lett. 2007 Oct 25;9(22):4651-3. doi: 10.1021/ol702148x. Epub 2007 Oct 2.
Org Lett. 2007.
PMID: 17910469
Alpha-keto esters can be converted into alpha-hydroxy acids in a single flask involving metal-catalyzed silylene transfer, 6pi-electrocyclization, Ireland-Claisen rearrangement, and hydrolysis. ...
Alpha-keto esters can be converted into alpha-hydroxy acids in a single flask involving metal-catalyzed silylene transfer, 6pi-electr …
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