Cell-free biosensors for rapid detection of water contaminants.
Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pastén PA, Matiasek SJ, Gaillard JF, Giedroc DP, Collins JJ, Lucks JB.
Jung JK, et al.
Nat Biotechnol. 2020 Dec;38(12):1451-1459. doi: 10.1038/s41587-020-0571-7. Epub 2020 Jul 6.
Nat Biotechnol. 2020.
PMID: 32632301
Free PMC article.
We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and sy …
We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (RO …