Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting.
Boselli D, Panigada M, Di Terlizzi S, Romanò M, Canonico E, Villa C, Minici C, van Anken E, Soprana E, Siccardi AG.
Boselli D, et al.
J Transl Med. 2023 Jul 23;21(1):495. doi: 10.1186/s12967-023-04353-7.
J Transl Med. 2023.
PMID: 37482614
Free PMC article.
Direct Virus-Sorting was performed on BD FACSAria Fusion cell sorter equipped with 4 lasers and a 100-mm nozzle, with 20 psi pressure and a minimal flow rate, validated using Megamix beads. RESULTS: Upon infection of cells with recombinant EEVs, at the first sorting step v …
Direct Virus-Sorting was performed on BD FACSAria Fusion cell sorter equipped with 4 lasers and a 100-mm nozzle, with 20 psi pressure and a …