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Page 1
The design of a quantitative western blot experiment.
Taylor SC, Posch A. Taylor SC, et al. Biomed Res Int. 2014;2014:361590. doi: 10.1155/2014/361590. Epub 2014 Mar 16. Biomed Res Int. 2014. PMID: 24738055 Free PMC article. Review.
Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detection, and the applicability of multiplexed target detection, the basic technique has remained essentially unchanged. In the past, western …
Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detectio …
A systematic approach to quantitative Western blot analysis.
Pillai-Kastoori L, Schutz-Geschwender AR, Harford JA. Pillai-Kastoori L, et al. Anal Biochem. 2020 Mar 15;593:113608. doi: 10.1016/j.ab.2020.113608. Epub 2020 Jan 31. Anal Biochem. 2020. PMID: 32007473 Free article. Review.
Attaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the user. Appropriate protein extraction method, electrophoresis, and transfer of proteins, immunodetection of blotted protein by antibodies, a …
Attaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the …
Western blot analysis.
Hirano S. Hirano S. Methods Mol Biol. 2012;926:87-97. doi: 10.1007/978-1-62703-002-1_6. Methods Mol Biol. 2012. PMID: 22975958
Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tissues exposed to nanoparticles or nanomaterials. ...In this chapter the whole process of western blot analysis, from sample prep …
Electrophoresis and the following western blot analysis are indispensable to investigate biochemical changes in cells and tiss …
Determination of Caspase Activation by Western Blot.
Chehade H, Fox A, Mor GG, Alvero AB. Chehade H, et al. Methods Mol Biol. 2021;2255:1-12. doi: 10.1007/978-1-0716-1162-3_1. Methods Mol Biol. 2021. PMID: 34033089
In the context of apoptosis, the proper analysis of western blot results depends on the understanding of the mechanisms and outcomes of caspase processing during the course of its activation. In this chapter, we describe the step-by-step methodology in the wester …
In the context of apoptosis, the proper analysis of western blot results depends on the understanding of the mechanisms and ou …
A defined methodology for reliable quantification of Western blot data.
Taylor SC, Berkelman T, Yadav G, Hammond M. Taylor SC, et al. Mol Biotechnol. 2013 Nov;55(3):217-26. doi: 10.1007/s12033-013-9672-6. Mol Biotechnol. 2013. PMID: 23709336 Free PMC article.
Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. ...This entails a necessary evolution of the procedures …
Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative met …
A quantitative western blot technique using TMB: Comparison with the conventional technique.
Ntoukas A, Niarchos A, Tsika AC, Mantzoukas S, Spyroulias GA, Poulas K. Ntoukas A, et al. Electrophoresis. 2021 Mar;42(6):786-792. doi: 10.1002/elps.202000306. Epub 2021 Jan 6. Electrophoresis. 2021. PMID: 33340122
Numerous molecular biological experiments performed throughout the world require the detection or quantification of a protein of interest. Western blotting is one of the most popular techniques used for this purpose and offers quantitative information with th …
Numerous molecular biological experiments performed throughout the world require the detection or quantification of a protein of interest. …
Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein.
Sigismund S, Polo S. Sigismund S, et al. Methods Mol Biol. 2016;1449:143-51. doi: 10.1007/978-1-4939-3756-1_6. Methods Mol Biol. 2016. PMID: 27613032 Review.
The simplest and more direct approach is to immunoprecipitate (IP) your target protein from cell lysate (stimulated and/or properly treated), followed by western blot analysis utilizing specific antibodies against Ub (see Subheading 3.1). ...This second method relie …
The simplest and more direct approach is to immunoprecipitate (IP) your target protein from cell lysate (stimulated and/or properly treated) …
Absolute Quantification of TGF-beta Signaling Proteins Using Quantitative Western Blot.
Deng D, Zi Z. Deng D, et al. Methods Mol Biol. 2022;2488:1-12. doi: 10.1007/978-1-0716-2277-3_1. Methods Mol Biol. 2022. PMID: 35347678
Here, we introduce how to measure the amount of TGF-beta signaling proteins using quantitative immunoblotting. In addition, we discuss how to convert the measurements of protein abundance to the quantities of absolute molecules per cell. ...
Here, we introduce how to measure the amount of TGF-beta signaling proteins using quantitative immunoblotting. In addition, we discus …
Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array.
Koch RJ, Barrette AM, Stern AD, Hu B, Bouhaddou M, Azeloglu EU, Iyengar R, Birtwistle MR. Koch RJ, et al. Sci Rep. 2018 Jul 27;8(1):11329. doi: 10.1038/s41598-018-29436-0. Sci Rep. 2018. PMID: 30054510 Free PMC article.
Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 ant …
Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, …
Western blot assay for quantitative and qualitative antigen detection in vaccine development.
Kumar S, Zheng H, Mahajan B, Kozakai Y, Morin M, Locke E. Kumar S, et al. Curr Protoc Microbiol. 2014 May 1;33:18.4.1-11. doi: 10.1002/9780471729259.mc1804s33. Curr Protoc Microbiol. 2014. PMID: 24789597
Immunological methods for quantitative measurement, antigenic characterization, and monitoring the stability of active immunogenic component(s) are a critical need in the vaccine development process. This unit describes an enhanced chemiluminescence-based western …
Immunological methods for quantitative measurement, antigenic characterization, and monitoring the stability of active immunogenic co …
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