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Showing results for expar
Search for rexpar instead (2 results)
Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example.
Reid MS, Le XC, Zhang H. Reid MS, et al. Angew Chem Int Ed Engl. 2018 Sep 10;57(37):11856-11866. doi: 10.1002/anie.201712217. Epub 2018 Aug 15. Angew Chem Int Ed Engl. 2018. PMID: 29704305 Review.
We highlight here recent advances in the exponential amplification reaction (EXPAR) for the detection of nucleic acids, proteins, enzyme activities, cells, and metal ions. ...
We highlight here recent advances in the exponential amplification reaction (EXPAR) for the detection of nucleic acids, proteins, enz …
Cas12a-assisted RTF-EXPAR for accurate, rapid and simple detection of SARS-CoV-2 RNA.
Hang XM, Wang HY, Liu PF, Zhao KR, Wang L. Hang XM, et al. Biosens Bioelectron. 2022 Nov 15;216:114683. doi: 10.1016/j.bios.2022.114683. Epub 2022 Sep 6. Biosens Bioelectron. 2022. PMID: 36088673 Free PMC article.
We, herein, reported the performance of a Cas12a-assisted RTF-EXPAR strategy for the identification of SARS-CoV-2 RNA. This assay combined the advantages of RTF-EXPAR with CRISPR-Cas12a can detect SARS-CoV-2 within 40 min, requiring only isothermal control. Particul …
We, herein, reported the performance of a Cas12a-assisted RTF-EXPAR strategy for the identification of SARS-CoV-2 RNA. This assay com …
Permethrin.
[No authors listed] [No authors listed] 2023 Sep 15. Drugs and Lactation Database (LactMed®) [Internet]. Bethesda (MD): National Institute of Child Health and Human Development; 2006–. 2023 Sep 15. Drugs and Lactation Database (LactMed®) [Internet]. Bethesda (MD): National Institute of Child Health and Human Development; 2006–. PMID: 30000442 Free Books & Documents. Review.
Electrochemical detection of the p53 gene using exponential amplification reaction (EXPAR) and CRISPR/Cas12a reactions.
Zhou S, Deng L, Dong J, Lu P, Qi N, Huang Z, Yang M, Huo D, Hou C. Zhou S, et al. Mikrochim Acta. 2023 Mar 4;190(4):113. doi: 10.1007/s00604-023-05642-0. Mikrochim Acta. 2023. PMID: 36869936
An improved electrochemical sensor has been developed for sensitive detection of the p53 gene based on exponential amplification reaction (EXPAR) and CRISPR/Cas12a. Restriction endonuclease BstNI is introduced to specifically identify and cleave the p53 gene, generating pr …
An improved electrochemical sensor has been developed for sensitive detection of the p53 gene based on exponential amplification reaction ( …
Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR.
Carter JG, Orueta Iturbe L, Duprey JHA, Carter IR, Southern CD, Rana M, Whalley CM, Bosworth A, Beggs AD, Hicks MR, Tucker JHR, Dafforn TR. Carter JG, et al. Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):e2100347118. doi: 10.1073/pnas.2100347118. Proc Natl Acad Sci U S A. 2021. PMID: 34400545 Free PMC article.
This involves the formation of an RNA/DNA heteroduplex whose selective cleavage generates a short DNA trigger strand, which is then rapidly amplified using the exponential amplification reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the R …
This involves the formation of an RNA/DNA heteroduplex whose selective cleavage generates a short DNA trigger strand, which is then rapidly …
Sequence dependence of isothermal DNA amplification via EXPAR.
Qian J, Ferguson TM, Shinde DN, Ramírez-Borrero AJ, Hintze A, Adami C, Niemz A. Qian J, et al. Nucleic Acids Res. 2012 Jun;40(11):e87. doi: 10.1093/nar/gks230. Epub 2012 Mar 13. Nucleic Acids Res. 2012. PMID: 22416064 Free PMC article.
Therefore, new computational tools are needed to facilitate assay design. In the isothermal EXPonential Amplification Reaction (EXPAR), template sequences with similar thermodynamic characteristics perform very differently. ...Since polymerases have a higher affinity for p …
Therefore, new computational tools are needed to facilitate assay design. In the isothermal EXPonential Amplification Reaction (EXPAR …
Design and synthesis of DNA hydrogel based on EXPAR and CRISPR/Cas14a for ultrasensitive detection of creatine kinase MB.
Chen M, Zhang J, Peng Y, Bai J, Li S, Han D, Ren S, Qin K, Zhou H, Han T, Wang Y, Gao Z. Chen M, et al. Biosens Bioelectron. 2022 Dec 15;218:114792. doi: 10.1016/j.bios.2022.114792. Epub 2022 Oct 8. Biosens Bioelectron. 2022. PMID: 36242902
In this strategy, the designed CRISPR/Cas14a system can be activated by introducing complementary DNA (cDNA) derived from competitive dissociation and exponential amplification (EXPAR), which is positively correlated with creatine kinase isoenzyme (CK-MB) concentration. .. …
In this strategy, the designed CRISPR/Cas14a system can be activated by introducing complementary DNA (cDNA) derived from competitive dissoc …
A fluorescent amplification strategy for high-sensitive detection of 17 beta-estradiol based on EXPAR and HCR.
Wang Y, Zhao X, Zhang M, Sun X, Bai J, Peng Y, Li S, Han D, Ren S, Wang J, Han T, Gao Y, Ning B, Gao Z. Wang Y, et al. Anal Chim Acta. 2020 Jun 15;1116:1-8. doi: 10.1016/j.aca.2020.04.010. Epub 2020 Apr 6. Anal Chim Acta. 2020. PMID: 32389184
In this research, we constructed a fluorescence strategy for two-step amplification that combined two currently popular methods, exponential amplification reaction (EXPAR) and hybridization chain reaction (HCR). E2 competed with the complementary DNA (cDNA) to bind the apt …
In this research, we constructed a fluorescence strategy for two-step amplification that combined two currently popular methods, exponential …
A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase.
Zhang YP, Cui YX, Li XY, Du YC, Tang AN, Kong DM. Zhang YP, et al. Chem Commun (Camb). 2019 Jul 7;55(53):7611-7614. doi: 10.1039/c9cc03568k. Epub 2019 Jun 13. Chem Commun (Camb). 2019. PMID: 31192323
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). ...
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasen …
Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.
Srivastava P, Prasad D. Srivastava P, et al. 3 Biotech. 2023 Jun;13(6):200. doi: 10.1007/s13205-023-03628-6. Epub 2023 May 18. 3 Biotech. 2023. PMID: 37215369 Free article. Review.
LAMP, NASBA, SDA, RCA and RPA are some of the isothermal amplification techniques which are preferable for POC diagnostics. Furthermore, methods such as WGA, CPA, HDA, EXPAR, SMART, SPIA and DAMP were introduced for even more accuracy and robustness. ...
LAMP, NASBA, SDA, RCA and RPA are some of the isothermal amplification techniques which are preferable for POC diagnostics. Furthermore, met …
117 results