Thermal inactivation of COVID-19 specimens improves RNA quality and quantity.
Hemati M, Soosanabadi M, Ghorashi T, Ghaffari H, Vahedi A, Sabbaghian E, Rasouli Nejad Z, Salati A, Danaei N, Kokhaei P.
Hemati M, et al.
J Cell Physiol. 2021 Jul;236(7):4966-4972. doi: 10.1002/jcp.30206. Epub 2020 Dec 11.
J Cell Physiol. 2021.
PMID: 33305832
Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab genes (p = .009, p = .032, respectively). Thermal inactivation elevated concentration and copy number of extracted-RNA, possibly through viral-c …
Significantly higher levels of RNA copy number were obtained in inactivated compared to the active samples for both N and ORF1ab gene …