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Page 1
Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Lam SS, et al. Nat Methods. 2015 Jan;12(1):51-4. doi: 10.1038/nmeth.3179. Epub 2014 Nov 24. Nat Methods. 2015. PMID: 25419960 Free PMC article.
Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of …
Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic e …
Defining Proximity Proteome of Histone Modifications by Antibody-mediated Protein A-APEX2 Labeling.
Li X, Zhou J, Zhao W, Wen Q, Wang W, Peng H, Gao Y, Bouchonville KJ, Offer SM, Chan K, Wang Z, Li N, Gan H. Li X, et al. Genomics Proteomics Bioinformatics. 2022 Feb;20(1):87-100. doi: 10.1016/j.gpb.2021.09.003. Epub 2021 Sep 30. Genomics Proteomics Bioinformatics. 2022. PMID: 34555496 Free PMC article.
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein complexes and protein-protein interactions. ...To address this limitation, we developed a new method to label proximal proteins of interest b …
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein c …
Genetic Screens Reveal FEN1 and APEX2 as BRCA2 Synthetic Lethal Targets.
Mengwasser KE, Adeyemi RO, Leng Y, Choi MY, Clairmont C, D'Andrea AD, Elledge SJ. Mengwasser KE, et al. Mol Cell. 2019 Mar 7;73(5):885-899.e6. doi: 10.1016/j.molcel.2018.12.008. Epub 2019 Jan 24. Mol Cell. 2019. PMID: 30686591 Free PMC article.
We found that BRCA2-deficient cells are selectively dependent on multiple pathways including base excision repair, ATR signaling, and splicing. We identified APEX2 and FEN1 as synthetic lethal genes with both BRCA1 and BRCA2 loss of function. BRCA2-deficient cells require …
We found that BRCA2-deficient cells are selectively dependent on multiple pathways including base excision repair, ATR signaling, and splici …
APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation.
Chen L, Li N, Zhang M, Sun M, Bian J, Yang B, Li Z, Wang J, Li F, Shi X, Wang Y, Yuan F, Zou P, Shan C, Wang J. Chen L, et al. Angew Chem Int Ed Engl. 2021 Nov 22;60(48):25346-25355. doi: 10.1002/anie.202108961. Epub 2021 Oct 27. Angew Chem Int Ed Engl. 2021. PMID: 34550632
Mammalian cell nuclei contain copper, and cancer cells are known to accumulate aberrantly high copper levels, yet the mechanisms underlying nuclear accumulation and copper's broader functional significance remain poorly understood. Here, by combining APEX2-based proximity …
Mammalian cell nuclei contain copper, and cancer cells are known to accumulate aberrantly high copper levels, yet the mechanisms underlying …
Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2.
Hung V, Udeshi ND, Lam SS, Loh KH, Cox KJ, Pedram K, Carr SA, Ting AY. Hung V, et al. Nat Protoc. 2016 Mar;11(3):456-75. doi: 10.1038/nprot.2016.018. Epub 2016 Feb 11. Nat Protoc. 2016. PMID: 26866790 Free PMC article.
This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engineered peroxidase, APEX2, is genetically targeted to a cellular region of interest. Upon the addition of hydrogen peroxide for 1 min …
This protocol describes a method to obtain spatially resolved proteomic maps of specific compartments within living mammalian cells. An engi …
Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Fazal FM, et al. Cell. 2019 Jul 11;178(2):473-490.e26. doi: 10.1016/j.cell.2019.05.027. Epub 2019 Jun 20. Cell. 2019. PMID: 31230715 Free PMC article.
We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of RNA using the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome as a resource, revealing ext …
We introduce APEX-seq, a method for RNA sequencing based on direct proximity labeling of RNA using the peroxidase enzyme APEX2. APEX- …
Identification of APEX2 as an oncogene in liver cancer.
Zheng R, Zhu HL, Hu BR, Ruan XJ, Cai HJ. Zheng R, et al. World J Clin Cases. 2020 Jul 26;8(14):2917-2929. doi: 10.12998/wjcc.v8.i14.2917. World J Clin Cases. 2020. PMID: 32775374 Free PMC article.
We included the TCGA liver cancer database in GSEA analysis based on the high and low APEX2 expression, showing the potential mechanisms of APEX2 in LIHC. ...CONCLUSION: APEX2 is overexpressed in LIHC, and the higher APEX2 level is associated with a wo …
We included the TCGA liver cancer database in GSEA analysis based on the high and low APEX2 expression, showing the potential mechani …
APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.
Naki M, Gourdomichali O, Zonke K, Kattan FG, Makridakis M, Kontostathi G, Vlahou A, Doxakis E. Naki M, et al. Int J Mol Sci. 2022 May 6;23(9):5189. doi: 10.3390/ijms23095189. Int J Mol Sci. 2022. PMID: 35563580 Free PMC article.
Despite these well-defined biological functions, little is known about its interacting partners and their unique molecular functions. Here, fusing an enhanced ascorbate peroxidase 2 (APEX2) biotin-labeling enzyme to DDIT4 combined with mass spectrometry, the proteins in th …
Despite these well-defined biological functions, little is known about its interacting partners and their unique molecular functions. Here, …
APEX2 Proximity Proteomics Resolves Flagellum Subdomains and Identifies Flagellum Tip-Specific Proteins in Trypanosoma brucei.
Vélez-Ramírez DE, Shimogawa MM, Ray SS, Lopez A, Rayatpisheh S, Langousis G, Gallagher-Jones M, Dean S, Wohlschlegel JA, Hill KL. Vélez-Ramírez DE, et al. mSphere. 2021 Feb 10;6(1):e01090-20. doi: 10.1128/mSphere.01090-20. mSphere. 2021. PMID: 33568455 Free PMC article.
Having validated the use of APEX2 in T. brucei, we next attempted to distinguish flagellar subdomains by fusing APEX2 to a flagellar membrane protein that is restricted to the flagellum tip, AC1, and another one that is excluded from the tip, FS179. Fluorescence mic …
Having validated the use of APEX2 in T. brucei, we next attempted to distinguish flagellar subdomains by fusing APEX2 to a fla …
294 results