Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1973 1
1974 1
1975 1
1976 1
1977 15
1978 58
1979 51
1980 54
1981 80
1982 140
1983 181
1984 241
1985 270
1986 311
1987 375
1988 548
1989 677
1990 747
1991 807
1992 697
1993 640
1994 591
1995 605
1996 672
1997 614
1998 632
1999 765
2000 964
2001 1092
2002 1080
2003 1206
2004 1267
2005 1148
2006 919
2007 821
2008 839
2009 755
2010 742
2011 846
2012 854
2013 712
2014 646
2015 600
2016 633
2017 522
2018 455
2019 452
2020 418
2021 370
2022 302
2023 234
2024 105

Text availability

Article attribute

Article type

Publication date

Search Results

26,178 results

Results by year

Filters applied: . Clear all
Page 1
Transfecting Macrophages.
Keller AA, Maeß MB, Schnoor M, Scheiding B, Lorkowski S. Keller AA, et al. Methods Mol Biol. 2018;1784:187-195. doi: 10.1007/978-1-4939-7837-3_18. Methods Mol Biol. 2018. PMID: 29761400
In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA. ...The presented electroporation protocol provides an effective …
In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of g …
Design of lipid nanoparticles for in vitro and in vivo delivery of plasmid DNA.
Kulkarni JA, Myhre JL, Chen S, Tam YYC, Danescu A, Richman JM, Cullis PR. Kulkarni JA, et al. Nanomedicine. 2017 May;13(4):1377-1387. doi: 10.1016/j.nano.2016.12.014. Epub 2016 Dec 28. Nanomedicine. 2017. PMID: 28038954
Lipid nanoparticles (LNPs) containing distearoylphosphatidlycholine (DSPC), and ionizable amino-lipids such as dilinoleylmethyl-4-dimethylaminobutyrate (DLin-MC3-DMA) are potent siRNA delivery vehicles in vivo. Here we explore the utility of similar LNP systems as transfection …
Lipid nanoparticles (LNPs) containing distearoylphosphatidlycholine (DSPC), and ionizable amino-lipids such as dilinoleylmethyl-4-dimethylam …
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology.
Colin B, Rocq N, Deprez B, Couturier C. Colin B, et al. J Vis Exp. 2019 Aug 8;(150). doi: 10.3791/59570. J Vis Exp. 2019. PMID: 31449254
Here we present an optimal protocol to perform ADE-based high-throughput plasmid transfection which makes it possible to reach an efficiency of up to 90% and a nearly 100% cotransfection in cotransfection experiments. ...The described software-based control of ADE …
Here we present an optimal protocol to perform ADE-based high-throughput plasmid transfection which makes it possible to reach …
Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production.
Yang S, Zhou X, Li R, Fu X, Sun P. Yang S, et al. Curr Protoc Chem Biol. 2017 Sep 14;9(3):147-157. doi: 10.1002/cpch.25. Curr Protoc Chem Biol. 2017. PMID: 28910855
PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based transfection is affected by numerous factors, including the way the PEI/DNA complex is prepared, the ratio of PEI to DNA, …
PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based …
DNA Targeting Sequence Improves Magnetic Nanoparticle-Based Plasmid DNA Transfection Efficiency in Model Neurons.
Vernon MM, Dean DA, Dobson J. Vernon MM, et al. Int J Mol Sci. 2015 Aug 17;16(8):19369-86. doi: 10.3390/ijms160819369. Int J Mol Sci. 2015. PMID: 26287182 Free PMC article.
Efficient non-viral plasmid DNA transfection of most stem cells, progenitor cells and primary cell lines currently presents an obstacle for many applications within gene therapy research. From a standpoint of efficiency and cell viability, magnetic nanopartic …
Efficient non-viral plasmid DNA transfection of most stem cells, progenitor cells and primary cell lines currently pres …
Effects of lyoprotectants on long-term stability and transfection efficacy of lyophilized poly(lactide-co-glycolide)-graft-polyethylenimine/plasmid DNA polyplexes.
Woo J, Lee JS. Woo J, et al. Nanomedicine (Lond). 2021 Jun;16(15):1269-1280. doi: 10.2217/nnm-2021-0065. Epub 2021 May 28. Nanomedicine (Lond). 2021. PMID: 34044608 Free PMC article.
Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polyplexes prepared from poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) and plasmid DNA encoding green fluorescent protein …
Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polypl …
A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications.
Raymond C, Tom R, Perret S, Moussouami P, L'Abbé D, St-Laurent G, Durocher Y. Raymond C, et al. Methods. 2011 Sep;55(1):44-51. doi: 10.1016/j.ymeth.2011.04.002. Epub 2011 Apr 24. Methods. 2011. PMID: 21539918
As with most transfection reagents, PEI-mediated transfection involves the formation of nanoparticles (polyplexes) which are obtained by its mixing with plasmid DNA. ...To avoid variations in transfection efficiency and productivity that arise f …
As with most transfection reagents, PEI-mediated transfection involves the formation of nanoparticles (polyplexes) which are o …
Development of Polymer-Lipid Hybrid Nanoparticles for Large-Sized Plasmid DNA Transfection.
Maeki M, Uno S, Sugiura K, Sato Y, Fujioka Y, Ishida A, Ohba Y, Harashima H, Tokeshi M. Maeki M, et al. ACS Appl Mater Interfaces. 2024 Jan 17;16(2):2110-2119. doi: 10.1021/acsami.3c14714. Epub 2023 Dec 23. ACS Appl Mater Interfaces. 2024. PMID: 38141015
RNA and DNA delivery technologies using lipid nanoparticles (LNPs) have advanced significantly, as demonstrated by their successful application in mRNA vaccines. ...However, a significant challenge remains in achieving efficient transfection, as the size of the deli …
RNA and DNA delivery technologies using lipid nanoparticles (LNPs) have advanced significantly, as demonstrated by their successful a …
A method for plasmid purification directly from yeast.
Singh MV, Weil PA. Singh MV, et al. Anal Biochem. 2002 Aug 1;307(1):13-7. doi: 10.1016/s0003-2697(02)00018-0. Anal Biochem. 2002. PMID: 12137773
A rapid technique for purifying plasmids from yeast Saccharomyces cerevisiae is described that yields high-quality DNA suitable for bacterial transformation, yeast transformation, and direct DNA sequencing. ...Both yeast single-copy CEN/ARS and high-copy 2mic …
A rapid technique for purifying plasmids from yeast Saccharomyces cerevisiae is described that yields high-quality DNA suitabl …
Cytoplasmic transport and nuclear import of plasmid DNA.
Bai H, Lester GMS, Petishnok LC, Dean DA. Bai H, et al. Biosci Rep. 2017 Nov 29;37(6):BSR20160616. doi: 10.1042/BSR20160616. Print 2017 Dec 22. Biosci Rep. 2017. PMID: 29054961 Free PMC article. Review.
Productive transfection and gene transfer require not simply the entry of DNA into cells and subsequent transcription from an appropriate promoter, but also a number of intracellular events that allow the DNA to move from the extracellular surface of the cell …
Productive transfection and gene transfer require not simply the entry of DNA into cells and subsequent transcription from an …
26,178 results
You have reached the last available page of results. Please see the User Guide for more information.